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[Abstract = "Addiction to drugs is one of the significant problems in many countries. Opium is a kind of drug that its use goes back to many years ago. Dopamine and serotonin are neurotransmitters involved in the process of addiction. As a result, this study aimed to investigate the blood levels of serotonin, dopamine and physical fitness factors in active and inactive men addicted to opium during their rehabilitation period. This study is a descriptive and Causal after the occurrence. A total of 34 men addicted to opium referring to rehabilitation center had been admitted voluntarily as samples were available in the study. According to a questionnaire answered by them, 16 of them did exercise during their rehabilitation period (active group) and 18 of them did not do any physical activity during this period (inactive group). 5 cc of peripheral blood samples were obtained from both groups and were tested their aerobic capacity, muscular endurance, flexibility, body composition, blood pressure and heart rate. Statistical analysis showed that blood levels of serotonin and dopamine in active group were significantly more than inactive group (p<0.05). In addition, the active group compared with the inactive group had low body fat percent, more muscular endurance, more flexibility, and low resting heart rate (p<0.05). According to the findings, we can conclude that low physical activity but as regular walking can affect blood levels of serotonin and dopamine, and health-related fitness factors of addicted men and as a non-drug treatment is helpful."]